Part:BBa_K2107002:Design

Platelet Derived Growth Factor-B M3

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part contains the same active site as PDGF-B isoform 1, but has been shortened to remove unnecessary flanking regions and decrease cost on the cell chassis to increase yield a protein folding efficiency.

Source

TAGAGAAAGAGGGGACAAACCAGTATGATTGCGGAATGCAAAACCCGCACCGAAGTGTTTGAAATTAGCCGCCGCCTGATTGATCGCACCAACGCGAACTTTCTGGTGTGGCCGCCGTGCGTGGAAGTGCAGCGTTGCAGCGGCTGCTGCAACAACCGCAACGTGCAGTGCCGCCCGACCCAGGTGCAACTGCGCCCGGTGCAGGTGCGCAAAATTGAAATTGTGCGCAAAAAACCGATTTTTAAAAAAGCGACCGTGACCCTGGAAGATCATCTGGCGTGCAAATGCGAAACCGTGCATCATCACCATCACCACTAATGA